Hello Michaela,
Thank you for your questions. You can add mandatory charges by adding Fees within Bookings>Taxes & Fees. Thus you can add Room Cleaning fee. This fee can be seen in the price breakdown on the checkout page.
As for one or two nights fee so you can manage such pricing by editing rates and adding pricing based on stay period. Unfortunately you can not highlight it at price breakdown as a separate fee but you can notify your customers about pricing peculiarities by adding description to room page.
best regards,
J. Davis